The Waters® ACQUIT Y UPLC® H-Class System delivers the flexibility of quaternary solvent blending with the advanced performance 
of UPLC® separations. The system’s holistic design is targeted for routine analysis and method development use and is perfectly 
suited for running both HPLC and UPLC applications while still realizing the improved resolution and sensitivity of UPLC separations. 
The system is comprised of a Quaternary Solvent Manager (QSM), a Sample Manager with Flow-Through Needle (SM-FTN) design,  
and offers a choice of column compartment products.  

SPECIFICATION

ACQUITY UPLC H-Class System Instrument Specifications

Flow precision  0.075% RSD or ±0.020 min SD, whichever is greater, based on six replicates (with i2 Valve) 60:40 H2O/MeOH via Auto•Blend Plus Technology, 0.5 mL/min, alkylphenone mix (5.0 μL injection volume), ACQUIT Y UPLC BEH C18, 1.7 μm, 2.1 x 50 mm, 35 °C ± 0.3 °C, UV @ 254 nm

Composition ripple (baseline noise)  <1.0 mAu (<0.1 mAU with optional 250.0 μL mixer) (with i2 Valve)  

A: H2O + 0.1% TFA, B: ACN + 0.1% TFA, 0.5 mL/min, ACQUIT Y UPLC BEH C18, 1.7 μm, 2.1 x 50 mm UV @ 214 nm, 10 mm analytical flow cell

Composition accuracy  ±0.5% absolute (full scale) from 5% to 90% from 0.5 to 2.0 mL/min (with i2 Valve) 

Degassed ACN/H2O (90:10), ACN/H2O (90:10) with caffeine at 12 mg/L concentration, back pressure 2000 psi, step gradient method, UV at 273 nm

Composition precision  <0.15% RSD or ±0.04 min SD, whichever is greater, based on six replicate injections (with i2 Valve) 

60:40 H2O/MeOH via Auto•Blend Plus Technology, 0.5 mL/min, alkylphenone mix (5.0 μL injection volume), ACQUIT Y UPLC BEH C18, 1.7 μm, 2.1 x 50 mm, 35 °C ± 0.3 °C, UV @ 254 nm 

Compressibility compensation   Automatic and continuous 

Priming  Wet priming can run at flow rates up to 4 mL/min

Pump seal wash  Equipped with an automated wash system to flush the rear of the high pressure seal and the plunger

Flow ramping  Range: 0.01 to 30.00 min to reach 2.0 mL/min 

Default: 0.45 min to reach 2.0 mL/min