1

Kinetic solubility determination for set of 1 to 4 compounds at 1 pH value (e.g., pH 7.4)

2

Thermodynamic solubility determination for set of 1 to 4 compounds at one pH value (e.g. pH 7.4): HPLC method development, 3 replicate samples at each pH value and 1 sample for stability studies

3

Log D determination for set of 1 to 4 compounds. Method: octanol/aqueous buffer pH 7.4, shake-flask, detection by HPLC-UV or LC-MS

4

Metabolic stability in liver microsomes (mouse, rat, human) for 1 compound: incubation for 1 h, at 5 time points, quantification of target compound by UPLC/MS/MS, positive, negative control, bioanalytical method development included

5

Plasma or blood stability (mouse, rat, human) for 1 compound: 37°C for 0-2 hours, 5 time points, positive, negative control, bioanalytical method development included

6

Plasma protein binding (mouse, rat, human) for 1 compound: RED device, 37°C for 4 hours, positive, negative control, bioanalytical method development included

7

Set of: a) metabolic stability in liver microsomes and b) plasma or blood stability for 1 compound, bioanalytical method development included

8

Set of: a) metabolic stability in liver microsomes and b) plasma protein binding for 1 compound, bioanalytical method development included

9

Set of: a) plasma or blood stability and b) plasma protein binding for 1 compound, bioanalytical method development included

10

Set of: a) plasma or blood stability; b) plasma protein binding and c) metabolic stability in liver microsomes for 1 compound, bioanalytical method development included