1

Tolerability (single dose toxicity) for 1 compound: 1 dose in 3 mice, observation period up to 24 h.

2

Pharmacokinetics for 1 compound: 1 dose in 3 mice, 5 time points

3

Excretion for 1 compound: 1 dose in 4 mice, urine collection pooled samples 1 h, 2 h, 3 h on "Hydrophobic sand"

4

Development of bioanalytical method (UPLC/MS) for quantitative determination of small molecules in blood plasma (evaluation of chromatographic and mass spectrometric parameters, sample preparation, selectivity, preliminary limit of quantification, detection linearity and preliminary working concentration range).

5

Bioanalysis of plasma samples by existing procedure (calibrators, QC samples)

6

Adaptation of plasma bioanalytical method for quantitative determination in mice urine (evaluation of chromatographic and mass spectrometric parameters, sample preparation, selectivity, preliminary limit of quantification, detection linearity and preliminary working concentration range)

7

Bioanalysis of urine samples by existing procedure (calibrators, QC samples)